Technology Platforms

X-ray Crystallography and SAXS

The lab is fully equipped for the crystallisation of proteins and their complexes using a variety of techniques. The following fluid-handling robotics systems are available for the formulation of custom crystallisation screens and for setting up crystallisation trays:

  • The Formulatrix Formulator can be used to create custom screening solutions from a number of stock solutions.
  • The Art Robbins Crystal Phoenix is used mainly to set up sitting drop crystallisation trials.
  • The Tecan Freedom EVO (supported by a Hercules foundation grant) is a very general liquid handling system. This system is supported by Hercules funding. Contact prof. Jan Steyaert for more information. 

An in-house X-ray generator (Rigaku MicroMax-007 HF) is available for screening and optimisation of crystals. It is equpped with an Oxford Cryostream system for data collection on frozen crystals, and a Saturn 944+ detector. For more demanding data collection projects, we have access to a number of world-class synchrotron sources, including ESRF, Soleil, DESY/PETRA and Diamond. Contact prof. Han Remaut for more information.

The X-ray generator is also equipped with a Rigaku BioSAXS 2000 Small-Angle X-Ray Scattering (SAXS) setup that enables solution scattering measurements on proteins and protein complexes to determine molecular weights and low-resolution shapes. Contact prof. Remy Loris for more information.

NMR Spectroscopy

The Jean Jeener NMR Centre is equipped with state-of-the-art 600 MHz and 800 MHz Varian spectrometers, supported by VIB, VUB and Hercules funds, as well as industrial collaborations. Contact Dr. Alex Volkov for more information.

Spectroscopy

In addition to standard UV absorption spectroscopy (Cary), we have a Jasco J-715 circular dichroism polarimeter and a Perkin-Elmer LS55 fluorimeters.

Surface Plasmon Resonance

Our trusty BiaCore 3000 system is used to study a wide range of biomolecular interactions.

Transmission Electron Microscopy

The transmission electron microscope (supported by a Hercules foundation grant) is used to visuallize cellular structures and protein aggregates. Contact dr. Rouslan Efremov for more information.

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